From pixel to actual size…?
Can anyone help me please.. I’m a biology student and I have a hard time thinking how to measure the actual size of my specimen in the microscope using a picture taken from it.. Is that possible using distance of pixels…????
Maybe, but the pixel size and density on each cameras sensor is unique to each camera brand and model number.
Samples:
Typical high quality P&S camera: Sensor 7.18 x 5.32 mm, 0.38 cm² with a pixel density of 26 MP/cm²
The camera I have attached to my microscope (or gross specimen scope) 23.6 x 15.8 mm, 3.72 cm² with a pixel density of 3.3 MP/cm²
In theory with the right formula, you could measure your specimen the way you propose, however there are more direct methods to get such measurements. Ask around the lab and see if there is such a measurement tool in someones drawer
Some oculars include a measurement scale and depending upon the objective lens you are using, you can calculate the size of your specimen
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