What are the field of view measurements for a Compound Light Microscope?
My homework wants the following:
In a compound light microscope…
1) What is the field of view for scan power in millimeters?
2) What is the field of view for low power in millimeters?
3) What is the field of view for high power in millimeters?
G. Determining Field-of-View Diameter
You may wish to estimate the size of the specimens (e.g., cells) you will see in lab. The best way to do this is with an ocular micrometer, a precision ocular lens insert that has a ruler etched into glass. The monocular scopes we use in the introductory courses are not so equipped, so we will use an alternative method based upon knowing the field-of-view diameter for your particular microscope. To do this, you must determine:
* the approximate diameter of your low magnification field-of-view for your particular microscope.
* the total magnification for each of your other objective lenses.
Knowing this for each objective lens, you can compare the size of the specimen against the known field diameter and make a reasonable esimate of size. This technique works for any microscope.
1. Obtain a slide scale and position it on your scope. A transparent metric ruler will work as well.
2. Bring it into focus using the 10x objective (100x total). The scale bars are increments of 1mm as shown in the figure below. Thus, a black bar = 0.5mm as does a space.
3. Move the slide such that the edge of an outside black bar is just tangent to the lighted field (see point “A” above).
4. Starting at that edge, estimate how many bars and spaces it takes to cross the field-of-view. You will probably have to estimate the last fraction of a space or bar. In the figure above, it is approximately 1.8mm wide.
5. Record your scope’s ID number and field diameter at 100x in your lab notebook for future reference.
6. Next, calculate the field width at 430x total magnification using the following formula (we refer to the 100x mag as “low power” and 430x as “high power”):
(low power mag/ high power mag) x low power field diameter (in mm)
For the example above,
(100 / 430) x 1.8 mm = 0.418 mm = 418 um (micrometers)
Note that the field diameter at high power is proportional to the ratio of the low to high power objectives. That is, as you increase magnification, the actual field of view becomes proportionally smaller.
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